CUET 2025 Biology Question Paper with Solutions: Free Download PDF

CUET 2025 Biology Question Paper with Solutions: The CUET 2025 exams were scheduled to take place from May 13 to June 3, 2025. CUET 2025 Biology Question Paper holds special importance for candidates who aspire to study undergraduate courses in life sciences, biotechnology, or medical sciences under the Common University Entrance Test (CUET) conducted by the National Testing Agency (NTA). This part contains questions based on Class 12 NCERT Biology basic concepts and is hence a crucial part for science stream aspirants. While the Biology paper has been concluded, this article will be updated with the complete question paper, solutions, and detailed CUET 2025 Biology Question Paper analysis .

CUET 2025 Biology Exam Pattern

The CUET 2025 exam pattern outlines the structure of the question paper, including the number of questions, marking scheme, and types of questions. Just like the CUET 2025 syllabus, understanding the exam pattern is essential for effective preparation, as it helps candidates prioritise key topics and manage their time efficiently during the exam.

CUET 2025 Biology Question Paper

This section will include all the questions asked in the Biology section, along with their solutions and explanations after the biology exam is over. This will help students analyse the type and difficulty level of questions and compare them with previous year trends for better insight.

CUET 2025 Biology Memory Based Questions

Memory-based questions from CUET 2025 help students understand the type of questions that appeared in the actual exam. They are useful for identifying important chapters, repeated concepts, and question formats. Practicing these can boost confidence and improve exam readiness.

Below are the questions that came in CUET Biology exam 2025:

1. What is the correct sequence of the malaria parasite’s life cycle in humans?

2. What is the correct sequence of events during HIV infection in the host cell?

3. Who coined the term 'Biodiversity'?

4. What is the chromosome number of a male honey bee (drone)?

5. Which disease is diagnosed using the Widal test?

6. Phenylketonuria is caused by a defect in which enzyme?

7. When did jawless fish originate?

8. Which cells are primarily attacked by the HIV/AIDS virus?

9. During oogenesis, the primary oocyte is arrested in which phase of meiosis?

10. Which hormone is released by hormone-releasing IUDs like Mirena?

11. Which hormones are commonly present in oral contraceptive pills?

CUET 2025 Biology Analysis 3rd June (Shift 2)

• Paragraph from ecosystem – productivity was asked

• Question from contraceptive methods was asked

• Question from embryo sac was asked

• Question from DNA isolation was asked

• Question related to insulin was asked

• Sex-linked recessive disease was asked

• Rhizobium related question was asked

• Paper was very easy, 90% questions were repeated

CUET 2025 Biology – 29th May (Shift 1) Analysis

Moderate Level | NCERT-Aligned, with Paragraph and Statement-Based MCQs

• In-situ and ex-situ conservation were again tested, continuing the trend.

• A question on Hershey-Chase experiment steps was included.

• A paragraph-based question came from biotechnology principles.

• Human health and disease included barrier methods via a paragraph.

• The definition of ecological niche was directly asked.

• Antigen-antibody interaction was a straightforward conceptual question.

• Microbes in human welfare had a match-the-following type question.

• A menstrual cycle phase-based question was asked.

• Spermatogenesis involved a statement-based MCQ.

• Fertilization included an acrosome-related question.

• Hormone-releasing IUDs (IVPs) were tested conceptually.

• Biodiversity summit date was included in a factual question.

CUET 2025 Biology – 28 May (Shift 1) Analysis

Easy Paper with Emphasis on Reproductive Biology and Microbial Processes

• The overall difficulty level was easy, favoring students thorough with NCERT concepts.

• The disease chapter was significant, including questions on the barrier method for contraception.

• Malaria cycle-based questions tested knowledge of vector-borne disease transmission.

• A sequencing-type question on HIV tested understanding of viral life cycle stages.

• Human Evolution questions involved a match-the-following format.

• Microbes in Human Welfare featured through questions on STP (Sewage Treatment Plant).

• Biotechnology questions focused on insulin production and genetic applications.

• Ecosystem questions emphasized decomposition and nutrient cycling.

• Zygote Intrafallopian Transfer (ZIFT) was tested in reproductive technology.

• Various contraceptive methods were covered, particularly hormonal and barrier methods.

• Spermatogenesis and oogenesis were tested in detail.

• Male accessory ducts were covered with questions on anatomy and function.

CUET 2025 Biology – 27 May (Shift 2) Analysis

Straightforward NCERT-Based Paper with Emphasis on Genetics and Reproduction

• Human reproduction included questions on menstrual cycle, male accessory glands, and spermatogenesis.

• Reproductive health topics like IVF, MTP, and IUDs were featured.

• Genetics questions covered prototrophism, incomplete dominance, and autosomal recessive disorders.

• Molecular biology topics included nucleosides and transcription units.

• A question on HIV and AIDS was asked from the human health and disease chapter.

• Biotechnology was covered through questions on EcoRI, gene therapy, and rDNA technology.

• GEAC (Genetic Engineering Appraisal Committee) full form was tested.

• Ecology questions included exponential population growth, population interactions, and Lindemann’s law.

CUET 2025 Biology – 26 May (Shift 2) Analysis

Mixed Question Formats Focused on NCERT Concepts

• Embryo sac structure and gamete formation were tested.

• A direct question on GIFT (Gamete Intra-Fallopian Transfer) was included.

• Genetics covered test cross and gel electrophoresis (sequence arrangement).

• Biotechnology questions included Bt Cry1Ab gene and genetically modified crops.

• Five-question passage on immunity types, immune cells, and roles.

• Another passage-based set tested understanding of biofertilizers like Rhizobium and cyanobacteria.

• Ecology featured population pyramid and standing crop questions.

CUET 2025 Biology – 23 May (Shift 2) Analysis

Conceptual NCERT Questions with Ecology and Terminology Emphasis

• The term ‘biodiversity’ and its origin was asked.

• Metagenesis and sexually transmitted diseases were tested through conceptual questions.

• A paragraph-based question on antibiotics featured.

• A sequencing question tested steps in gel electrophoresis.

• The male honey bee's chromosome number was tested.

• Developmental stages of pollen grain were arranged in sequence.

CUET 2025 Biology – 22 May (Shift 1) Analysis

NCERT-Driven Factual Questions Across Core Chapters

• Paper difficulty was easy to moderate.

• A passage-based question format was used, consistent with Physics and Chemistry papers.

• Microbes in Human Welfare featured through industrial and medical use questions.

• A direct question from Human Evolution was asked.

• Reproductive biology tested hormones secreted by placenta.

• Vidal Test (diagnostic tool) featured in disease-related questions.

• One question tested sequencing knowledge from genetics or evolution.

CUET 2025 Biology – 22 May (Shift 2) Analysis

Factual, NCERT-Aligned Biology Questions

• Questions were simple and high-scoring.

• Topics included seed dormancy, plant hormones, placenta functions, and stages of human reproduction.

• DNA replication enzyme was tested.

• The entire paper remained well within the NCERT framework.

CUET 2025 Biology – 21 May (Shift 1) Analysis

NCERT-Centric Questions from Reproduction, Genetics, and Human Health

• Overall paper was easy to moderate in difficulty.

• Dominant questions were from Human Reproduction, Genetics, and Biotechnology.

• Spermatogenesis and placental hormones were asked with direct NCERT references.

• Mendelian disorders like phenylketonuria and thalassemia were tested.

• Biotechnology featured questions on PCR and bioreactors.

• Immune system components like B cells and T cells were tested in detail.

• Ecology was lightly covered, with a single question on population attributes.

CUET 2025 Biology – 21 May (Shift 2) Analysis

Balanced Mix of Reproduction and Biotechnology with Some Ecology

• Questions were largely factual and straight from NCERT.

• Pollination mechanisms and double fertilization were asked.

• IVF and IUD-related questions were included from reproductive health.

• rDNA technology and Bt cotton gene-related questions were present.

• Human evolution and homologous organs were part of the paper.

• The ecological pyramid and energy flow were tested.

• One question on ex situ conservation added diversity.

CUET 2025 Biology – 20 May (Shift 1) Analysis

Paper Emphasized Core Chapters with Diagram-Based MCQs

• Students reported the paper to be very easy and NCERT-based.

• Human reproductive system labeling questions were included.

• Mendel’s laws and dihybrid cross traits were directly asked.

• Immunology featured antigens, antibodies, and allergy mechanisms.

• Microbes in industrial use (like LAB and Streptococcus) were covered.

• DNA packaging and chromatin structure were asked from molecular biology.

• Biogeochemical cycles and ecological succession featured in ecology.

CUET 2025 Biology – 20 May (Shift 2) Analysis

Direct Conceptual Questions with Focus on Reproduction and Ecology

• Double fertilization and embryo sac components were questioned.

• Hormonal contraception and IVF were among reproductive health topics.

• Molecular biology questions included RNA types and replication machinery.

• Population ecology and carrying capacity were tested.

• Disease-related questions included AIDS transmission and symptoms.

• Terminologies like totipotency and apomixis were included.

• Passage-based question featured mutualism and predation in ecosystems.

CUET 2025 Biology – 19 May (Shift 1) Analysis

Scoring Paper with Emphasis on Human Physiology and Molecular Biology

• The paper was easy and heavily NCERT-focused, as reported by students.

• Human Physiology topics like digestion, respiration, and excretion were asked in factual MCQ format.

• Biotechnology questions included tools like restriction enzymes and gel electrophoresis procedures.

• Transcription, translation, and post-transcriptional modifications were covered under molecular biology.

• Cell biology questions tested the structure and function of organelles such as lysosomes and mitochondria.

• Immunity-based questions involved types of antibodies and vaccination principles.

• Reproductive biology had questions related to menstrual cycle and gametogenesis.

CUET 2025 Biology – 19 May (Shift 2) Analysis

Application-Oriented Biology MCQs Based on NCERT Chapters

• Overall difficulty level was moderate; questions focused on direct application of NCERT theory.

• Evolution and genetics topics included Hardy-Weinberg principle and pedigree analysis.

• Plant physiology section asked about transpiration pull, osmosis, and stomatal movement.

• Reproductive health questions involved in-vitro fertilization and hormonal control of reproduction.

• Two assertion-reason type questions tested conceptual depth in physiology and ecology.

• One passage-based question covered biodiversity hotspots and conservation strategies.

• Disease and immunity chapter tested knowledge on allergens and autoimmune diseases.

CUET 2025 Biology – 17 May (Shift 1) Analysis

NCERT-Based Questions from Physiology, Genetics, and Reproduction

• Most students rated the paper as easy to moderate.

• Genetics featured inheritance-based problems and examples of polygenic inheritance.

• Plant physiology was tested with focus on photoperiodism and vernalization.

• Spermatogenesis and hormonal control were central to reproductive biology questions.

• DNA fingerprinting and PCR-based techniques were tested under biotechnology.

• Human diseases like malaria and typhoid were covered with emphasis on causative agents.

• Ecology included direct questions on trophic levels and energy pyramid patterns.

CUET 2025 Biology – 17 May (Shift 2) Analysis

Straightforward Questions from Immunity, Evolution, and Environment

• Paper was easy with a major share of questions being memory-based.

• Passive and active immunity were tested along with B-cells and T-cells functions.

• Evolution-related questions focused on fossil evidence and geological timescale.

• Questions from Human Health and Disease included roles of histamines and interferons.

• Plant hormones like gibberellins and auxins were covered under plant growth and development.

• Environment section had questions on global warming, greenhouse gases, and CFCs.

• Biotechnological techniques like cloning vectors and restriction digestion were also included.

CUET 2025 Biology – 16 May (Shift 1) Analysis

Easy Biology Paper with Emphasis on NCERT Concepts and Applied Biology

• Overall paper was easy, heavily favoring those familiar with NCERT textbook content.

• Evolution-based question on jawless fishes like lampreys and hagfishes was included.

• Seed biology was tested with a conceptual MCQ on albuminous vs. non-albuminous seeds.

• A human health-related question tested basic antibiotic awareness.

• Amniocentesis was asked under human reproduction, checking knowledge of prenatal diagnostic methods.

• Trichoderma, a bio-control fungus, featured in applied microbiology.

• Cry1Ab gene-related passage tested understanding of genetically modified crops.

CUET 2025 Biology – 16 May (Shift 2) Analysis

Conceptual and NCERT-Aligned Paper with Assertion-Reason Format

• Paper was easy to moderate; most questions were directly picked from NCERT.

• Diagrams and flowchart-based questions tested visual memory and conceptual clarity.

• Human physiology, genetics, and biotechnology were dominant sections.

• Assertion-Reason type questions added depth, especially in ecology and physiology.

• Several questions required applying biology to real-life and societal contexts.

• Factual recall was strong with questions from microbial applications and environmental biology.

• Students found the paper manageable with good time efficiency due to its simplicity.

CUET 2025 Biology – 15 May (Shift 1) Analysis

Straightforward and Factual Biology Paper with Direct NCERT Linkages

• Population pyramid interpretation was tested with easy demographic-based questions.

• Lac Operon model featured in genetics, testing understanding of prokaryotic gene regulation.

• AIDS virus question focused on CD4+ T cell targeting by HIV.

• Reproductive biology tested oogenesis stages, especially arrest at diplotene phase.

• Pisces and starfish feeding behavior were questioned in ecological context.

• Hormonal IUDs and their mechanism were part of contraceptive biology section.

• Birth control pill hormone content was asked under hormonal regulation.

CUET 2025 Biology – 15 May (Shift 2) Analysis

Balanced Mix of Ecology, Genetics, Reproduction, and Biotechnology

• Fruit formation without fertilization and population fertilization rates were key reproduction topics.

• Mendelian inheritance and genetic disorders featured under genetics.

• Direct questions on diseases like sickle cell anemia and hemophilia were present.

• Species-Area Curve and biodiversity conservation strategies appeared under ecology.

• Brood parasitism of cuckoo birds in crow nests was asked in ecological interactions.

• Hormonal cycles and sperm movement through testicular lobules featured in human reproduction.

• DNA ligase function and IUCDs were asked in biotechnology and reproductive health.

• Five match-the-column questions covered diverse biology topics, making it scoring.

• Two comprehension passages tested concepts on biodiversity and human health.

CUET 2025 Biology – 14 May (Shift 1) Analysis

Moderate Difficulty Paper with NCERT-Rooted Concepts

• Match the following questions included topics from reproductive health and biotechnology.

• Diagram-based question on pollen grain structure assessed familiarity with NCERT illustrations.

• Lac Operon and gene expression regulation was asked again, indicating a recurring trend.

• Inheritance pattern of hemophilia tested X-linked genetic disorder understanding.

• Ecology-based questions focused on species interaction types like mutualism and parasitism.

• Several assertion-reason questions covered biotechnology, immunity, and microbes in industry.

• Overall paper tested both factual memory and ability to apply biological knowledge.

CUET 2025 Biology – 14 May (Shift 2) Analysis

Fact-Focused and Scoring Paper with High NCERT Overlap

• Human reproductive system and menstrual cycle received strong focus with 3–4 questions.

• A question on Bt cotton gene confirmed GM crop awareness.

• Hardy-Weinberg principle and allele frequency-based MCQ was included under evolution.

• Sickle cell anemia question tested Mendelian genetics and codominance.

• Biomolecules section included a conceptual question on enzyme specificity.

• Ecological succession and biogeochemical cycles were addressed in 1–2 questions.

• A passage-based question tested comprehension of biodiversity hotspots and conservation strategies.

CUET 2025 Biology – 13 May (Shift 1) Analysis

Conceptual, NCERT-Focused Biology Paper with Moderate Difficulty

• The paper leaned more towards conceptual understanding than tricky numerical or analytical questions.

• Genetics included questions on Mendelian disorders, chromosomal abnormalities, and polygenic inheritance.

• Molecular biology was tested through multiple questions on transcription and translation, with practical life applications.

• Biodiversity and conservation appeared as a passage question, emphasizing in-situ and ex-situ strategies.

• Double fertilization was tested via a dedicated paragraph, showing the importance of plant reproduction.

• Evolution-based questions included homologous and analogous organs; one question on geological time periods was reportedly outside the syllabus.

• A question on the EcoRI enzyme from biotechnology tested familiarity with restriction enzymes.

CUET 2025 Biology – 13 May (Shift 2) Analysis

NCERT-Based, Factual Biology Questions with a Balanced Syllabus Coverage

• Human physiology dominated the section with questions on digestion, circulation, and respiration.

• Mendelian ratio-based numericals tested conceptual depth in the genetics and evolution section.

• Ecology and environment questions were easy and direct, focusing on fundamental ideas.

• Plant physiology featured topics like photosynthesis and mineral nutrition, with moderate challenge.

• Biotechnology and its Applications section had simple, real-life application-based questions.

• Cell structure and functions questions focused on organelles and transport, presented conceptually.

• Overall, it was an easy to moderate paper where NCERT textbook preparation proved sufficient.

Also Read,

• CUET 2025 PYQs

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• CUET 2025 Mock Test