CUET Biology Question Paper Analysis 2025 (Out), Paper Review & Difficult-level

CUET 2025 Biology Question Paper Analysis: The CUET 2025 Biology exam was conducted between May 13 and June 3, 2025, as part of the Common University Entrance Test (CUET) for undergraduate admissions to central and participating universities across India. As a core domain subject for life sciences aspirants, the Biology paper aimed to evaluate conceptual understanding, factual recall, and application-based thinking aligned with the NCERT Class 12 syllabus.

This Story also Contains

1. CUET 2025 Biology Exam Overview
2. CUET 2025 Biology Question Paper Analysis
3. Why Analysing the CUET 2025 Biology Paper is Important?

Now that the exam is over, this article presents a detailed CUET 2025 Biology question paper analysis, including chapter-wise weightage, question types, difficulty level, and a comparison with previous years’ trends. This analysis will help candidates assess their performance and offer future aspirants valuable insights for targeted preparation.

CUET 2025 Biology Exam Overview

The Biology section in CUET 2025 evaluates a candidate’s grasp of essential topics from both Botany and Zoology, with a blend of memory-based and concept-oriented questions. It holds significant weightage for students aiming to pursue life sciences, biotechnology, and health sciences at the undergraduate level.

Below is the CUET 2025 exam pattern followed for CUET 2025 Biology:

CUET 2025 Biology Question Paper Analysis

The CUET 2025 Biology Question Paper Analysis provides a detailed breakdown of the exam's difficulty level, important topics, and question patterns. Based strictly on the NCERT syllabus, the CUET Biology paper 2025 focused on conceptual clarity, application-based questions, and factual recall, making it crucial for students aiming for high scores in the CUET 2025 exam.

CUET 2025 Biology Analysis 3rd June (Shift 2)

• Paragraph from ecosystem – productivity was asked

• Question from contraceptive methods was asked

• Question from embryo sac was asked

• Question from DNA isolation was asked

• Question related to insulin was asked

• Sex-linked recessive disease was asked

• Rhizobium related question was asked

• Paper was very easy, 90% questions were repeated

CUET 2025 Biology Memory Based Questions

Memory-based questions from CUET 2025 help students understand the type of questions that appeared in the actual exam. They are useful for identifying important chapters, repeated concepts, and question formats. Practicing these can boost confidence and improve exam readiness.

Below are the questions that came in CUET Biology exam 2025:

1. What is the correct sequence of the malaria parasite’s life cycle in humans?

2. What is the correct sequence of events during HIV infection in the host cell?

3. Who coined the term 'Biodiversity'?

4. What is the chromosome number of a male honey bee (drone)?

5. Which disease is diagnosed using the Widal test?

6. Phenylketonuria is caused by a defect in which enzyme?

7. When did jawless fish originate?

8. Which cells are primarily attacked by the HIV/AIDS virus?

9. During oogenesis, the primary oocyte is arrested in which phase of meiosis?

10. Which hormone is released by hormone-releasing IUDs like Mirena?

11. Which hormones are commonly present in oral contraceptive pills?

CUET 2025 Biology – 29th May (Shift 1) Analysis

Moderate Level | NCERT-Aligned, with Paragraph and Statement-Based MCQs

• In-situ and ex-situ conservation were again tested, continuing the trend.

• A question on Hershey-Chase experiment steps was included.

• A paragraph-based question came from biotechnology principles.

• Human health and disease included barrier methods via a paragraph.

• The definition of ecological niche was directly asked.

• Antigen-antibody interaction was a straightforward conceptual question.

• Microbes in human welfare had a match-the-following type question.

• A menstrual cycle phase-based question was asked.

• Spermatogenesis involved a statement-based MCQ.

• Fertilization included an acrosome-related question.

• Hormone-releasing IUDs (IVPs) were tested conceptually.

• Biodiversity summit date was included in a factual question.

CUET 2025 Biology - 28th May (Shift 1) Analysis

• The overall difficulty level was Easy, making it highly accessible for most candidates.

• The disease chapter contributed significantly with questions focused on Barrier Method concepts.

• Malaria Cycle questions tested understanding of the complete life cycle and transmission patterns.

• HIV-related questions appeared in a sequence format, requiring knowledge of viral progression stages.

• Human Evolution topics were presented in a matching format under the broader Evolution chapter.

• The microbes chapter included questions on STP (Sewage Treatment Plant) processes and applications.

• The biotechnology section featured Insulin-related questions, focusing on production and applications.

• The ecosystem chapter emphasized Decomposition processes and nutrient cycling concepts.

• ZIFT (Zygote Intrafallopian Transfer) appeared as a reproductive technology question.

• Contraceptive Methods were well-represented, covering various birth control techniques.

• Reproductive biology topics included detailed questions on both Oogenesis and Spermatogenesis processes.

• Male Accessory Ducts were specifically tested, focusing on anatomical structure and functions.

• The paper maintained consistency with NCERT-based concepts without requiring advanced interpretations.

• Question distribution favored reproductive biology and disease-related topics predominantly.

CUET 2025 Biology Test - 27th May (Shift 2) Analysis

• The paper was easy.

• Questions were asked about human reproduction, including male accessory glands, the menstrual cycle, and spermatogenesis.

• Questions were asked about reproductive health topics such as MTP, IVF, and IUDs.

• A question was asked about genetics, about prototrophy and incomplete dominance.

• A question was asked about equivalent genetic material.

• A question on autosomal recessive disease was asked.

• A question was asked about nucleosides.

• A question about transcription units was asked.

• A question on the topic of evolution and the geological time scale was asked.

• A question from the chapter on human health and disease, specifically related to HIV, was asked.

• A question related to biotechnology on ECOR1 was asked.

• A question was asked on the first recombinant DNA (rDNA) experiment.

• A question was asked about gene therapy.

• The full form of GEAC (Genetic Engineering Appraisal Committee) was asked.

• A question on exponential population growth was asked in ecology.

• A question on population interactions was asked.

• A question about Lindemann’s law was asked.

CUET 2025 Biology 26 May (Shift 2) Analysis

• One question was asked on the structure of the embryo sac (female gametophyte in flowering plants).

• A match-the-following question tested knowledge of hormone-releasing intrauterine devices.

• A direct question was asked on Gamete Intra-Fallopian Transfer (GIFT).

• Spermatogenesis was covered with a question on the process of sperm formation.

• A concept-based question on test cross appeared under genetics.

• Gel electrophoresis was assessed with a question involving DNA sequence arrangement.

• The Bt gene Cry1Ab featured in a question on genetically modified crops.

• A paragraph-based set of five questions tested understanding of the immune system, covering immunity types and cell roles.

• Another passage-based set focused on biofertilizers, with questions on Rhizobium and cyanobacteria.

• Ecology and population concepts were tested with questions on the population pyramid and the standing crop.

CUET 2025 Biology - 23 May (Shift 2) Analysis

• The paper was rated easy by most students.

• A question was asked about who coined the term 'biodiversity', testing terminology.

• Conceptual questions appeared from metagenesis and identification of STDs.

• A paragraph-based question came from the topic of antibiotics.

• A sequencing question on gel electrophoresis was included.

• Students were asked about the chromosome number of male honey bees.

• A question required arranging the pollen grain developmental stages in sequence.

CUET 2025 Biology - 22 May (Shift 1) Analysis

• The overall difficulty level of the Biology paper was easy to moderate.

• The paper included a passage-based question format, similar to the one used in Physics, Chemistry, and Mathematics sections.

• A question was asked from the chapter Microbes in Human Welfare, focusing on their role in industrial and medical applications.

• Human Evolution was tested through a direct factual question related to evolutionary concepts.

• Placenta-related hormones were part of the reproductive biology questions, emphasizing endocrine functions.

• A question based on the Vidal Test was included, checking students’ knowledge of disease diagnostics.

• One of the questions involved sequence arrangement, likely testing understanding of processes or classification in genetics or evolution.

• Overall, the questions were NCERT-based, with a mix of factual recall and conceptual understanding.

CUET 2025 Biology - 22 May (Shift 2) Analysis

• The Biology section was easy and highly scoring.

• Questions were mostly NCERT-based, covering topics like seed dormancy, plant hormones, placenta, DNA replication enzymes, and stages of human reproduction.

CUET 2025 Biology– 20 May (Shift 1) Analysis

• Overall Difficulty Level: Easy

• Most students were able to attempt more than 45 questions confidently due to the familiarity and simplicity of the topics.

• Ecology and Environment topics dominated the paper with questions on sacred groves, productivity units, bell-shaped pyramids, and ecosystem-based paragraphs.

• The Rivet Popper Hypothesis was directly asked in the ecology section.

• Questions from reproduction included topics such as spermatogenesis and oogenesis, though the menstrual cycle (especially hormonal control) was notably not asked.

• A sequence-based question tested the process: "Yearn pore → Pollen".

• Two questions were asked about codons, testing basic genetic knowledge.

• Molecular biology concepts appeared in questions like "RNA Pol II → hnRNA → mRNA" and the structure/function of the embryo sac.

• Pollination and fertilization processes were included through the topic of the pollen tube.

• Questions related to population dynamics and ecosystem pyramids added depth to the ecology coverage.

CUET 2025 Biology - 20 May (Shift 2) Analysis

• The Biology paper was very easy, according to student feedback.

• All questions were based entirely on the NCERT syllabus.

• Main topics included Human Physiology, Genetics and Evolution, and Plant Physiology.

• No surprise or tricky questions were reported.

• The paper mainly consisted of one-liner factual questions.

• There were no analytical or diagram-based questions.

• The paper was high-scoring for those thorough with the NCERT.

• Time management was not a concern due to the simplicity of the questions.

CUET 2025 Biology — 16th May (Shift 1) Paper Analysis

The CUET 2025 Biology exam on 16th May (Shift 1) was easy, favoring students familiar with NCERT content.

• Evolution questions on jawless fishes were consistently asked.

• Plant Physiology was tested with questions on albuminous vs. non-albuminous seeds.

• A conceptual question on antibiotics reinforced human health knowledge.

• The amniocentesis-related question evaluated understanding of prenatal diagnosis.

• Trichoderma, an ecologically significant fungal genus, is featured in applied biology.

• Crygene-based passage tested comprehension and real-life application skills.

CUET 2025 Biology — 16th May (Shift 2) Analysis

The CUET 2025 Biology paper for 16th May (Shift 2) leaned towards an easy to moderate difficulty level, heavily grounded in the NCERT syllabus. Candidates familiar with NCERT found the questions straightforward and approachable.

• Human Physiology, Genetics, Ecology, and Biotechnology were the dominant topics, with multiple questions framed from these areas.

• The exam featured visual-based questions, including diagrams and flowcharts, assessing students' ability to interpret biological illustrations accurately.

• Assertion and Reasoning questions require a clear grasp of fundamental concepts and their practical applications.

• Several questions directly tested factual knowledge, rewarding students who had done multiple NCERT revisions.

• Application-based questions linked theoretical concepts to current environmental concerns, encouraging analytical reasoning.

• Overall, the section maintained a balance between conceptual clarity, memorization, and application-oriented questions, making it a scoring opportunity for well-prepared students.

CUET 2025 Biology — 15th May (Shift 1) Analysis

The CUET 2025 Biology paper for Shift 1 on 15th May was categorized as easy and concept-driven.

• Demography concepts are tested through a Population Pyramid diagram question.

• Lac Operon model in gene regulation is featured in molecular biology.

• AIDS-related question focused on CD4+ T-lymphocytes and immune system targeting.

• Reproductive Biology covered Oogenesis arrest during the diplotene stage of Prophase I.

• Ecology included questions on the feeding mechanisms of Piscaster (starfish).

• Contraceptive methods such as hormone-releasing IUCDs and oral pills were included.

CUET 2025 Biology — 15th May (Shift 2) Analysis

Shift 2 Biology paper on 15th May had a balanced and straightforward question pattern.

• Reproductive Biology questions on parthenocarpy and population fertilization rate calculations.

• Mendelian inheritance and gene disorders formed the core of Genetics questions.

• Ecology concepts like the Species-Area Relationship and brood parasitism (Cuckoo-Crow interaction) were featured.

• Detailed questions on human reproduction and hormonal regulation (FSH, LH, Estrogen, Progesterone).

• DNA Ligase enzyme function in replication and repair was tested.

• Family planning methods through IUCDs and hormonal contraceptives appeared.

• Match the following types of questions covered enzymes, hormones, anatomy, and diseases.

• Comprehension passages on biodiversity conservation and disease management were included.

CUET 2025 Biology — 14th May (Shift 1) Analysis

The Biology paper on 14th May (Shift 1) was designed with an Easy to Moderate level, heavily reliant on NCERT content.

• The diseases topic featured passage-based questions on primary and secondary lymphoid organs like the thymus, spleen, and lymph nodes.

• Active and Passive Immunity concepts were frequently tested with clear-cut factual questions.

• Students faced questions on B-lymphocytes and T Lymphocytes, their functions, types, and roles in the immune system.

• Antibodies (Immunoglobulins) were covered extensively, including types like IgA, IgG, and their roles in immune responses.

• Innate and Acquired Immunity concepts were assessed through differentiating features and examples.

• Vaccination principles and memory cell functions in secondary immune response were also tested.

• Immunological disorders such as allergies, autoimmune diseases, and AIDS were part of the questionnaire.

• Direct questions on antigen-antibody interactions, including agglutination and neutralization, were asked.

• Most questions were factual, making it an accessible section for NCERT-focused students.

CUET 2025 Biology — 14th May (Shift 2) Paper Review

The 14th May (Shift 2) Biology paper was reported as very easy, strictly based on the NCERT textbooks.

• Almost all 50 questions were attempted by most students due to the direct and fact-based nature.

• Memory-based topics like seed dormancy, hormones, and plant hormone functions were featured.

• Human reproduction questions included placenta function and DNA polymerase in replication.

• Sequential stages of human reproduction were tested.

• A scoring opportunity for students thoroughly familiar with the NCERT content.

CUET 2025 Biology — 13th May (Shift 1) Analysis

The CUET 2025 Biology exam on 13th May (Shift 1) maintained a moderate difficulty level, focusing primarily on NCERT-based questions that tested conceptual understanding.

• Genetics questions revolved around Mendelian disorders, chromosomal abnormalities, and polygenic inheritance, evaluating students’ grasp on inheritance patterns and variations.

• Molecular Biology was emphasized with multiple questions on transcription, translation, and real-life applications of gene expression.

• A passage-based question assessed knowledge on biodiversity and conservation, specifically ex-situ and in-situ conservation strategies.

• Applied Biology topics like biogas plants and double fertilization were tested through comprehensive passage-based questions.

• Evolution concepts were assessed with questions on homologous and analogous organs, including an out-of-syllabus question on geological time scales.

• Biotechnology was tested with application-based questions, including a notable one on the EcoRI restriction enzyme used in genetic engineering.

• The exam aligned closely with the NCERT syllabus, making it approachable for well-prepared students.

CUET 2025 Biology — 13th May (Shift 2) Analysis

The CUET 2025 Biology paper in Shift 2 of 13th May was largely NCERT-centric with an easy to moderate difficulty level.

• Human Physiology questions were abundant, focusing on digestion, respiration, and circulation, testing factual recall.

• Genetics & Evolution had numerical problems on Mendelian ratios, reinforcing inheritance concepts.

• Ecology and Environment topics were straightforward, featuring direct factual questions.

• Plant Physiology included moderate questions on photosynthesis and mineral nutrition.

• Biotechnology and Its Applications section presented easy application-based questions.

• Conceptual understanding was checked through Cell Structure and Functions questions.

• Overall, the paper was highly scoring for students with thorough NCERT preparation.

Why Analysing the CUET 2025 Biology Paper is Important?

A thorough question paper analysis enables students, educators, and future aspirants to extract maximum learning from the exam. Here’s why it's essential:

• Identifies frequently tested chapters and high-weightage areas.

• Reveals the balance between factual and conceptual questions.

• Helps in gauging the difficulty level, improving performance prediction.

• Assists in self-evaluation, helping candidates spot strengths and weaknesses.

• Offers trend insights for future aspirants and coaching institutes to revise their strategies.

• Helps determine whether NCERT-only preparation was sufficient or not.